Correlation of PEG/(NH4)2SO4 Aqueous Two-phase Equilibrium Data and Modeling of Partition of Papain
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Abstract:
In order to investigate the partition of papain in aqueous two-phase systems, the liquid-liquid phase equilibrium data of the aqueous two-phase systems containing poly(ethylene glycol) (2000, 4000, 6000) /ammonium sulfate was measured by cloud point measurements at T=298.15 K. The experimental binodal data and liquid-liquid phase equilibrium data were correlated using the Merchuk, Othmer-Tobisa, and Bancroft equations. A model was established to describe the partition of papain in the PEG/(NH4)2SO4 aqueous two-phase system. The degree of correlation between the component concentrations and partition coefficients in different groups of the aqueous two-phase system was examined using relevant coefficients. The logarithm of the partition coefficient and the concentration difference between the two-phase components had a relatively high correlation; meanwhile, an empirical equation describing the papain partition coefficient and concentration difference between the aqueous two-phase components was developed. The mean relative deviation between the experimental and calculated papain partition coefficients was less than 10%, and was also less than the values from similar models. This confirmed that the established model revealed a good correlation between papain in PEG/(NH4)2SO4aqueous two-phase system and could be used for calculation of papain partition coefficients. The result provided references for engineering calculations of papain partition coefficients in aqueous two-phase systems and design of aqueous two-phase extraction processes.
