Abstract:The furazolidone metabolite 3-amino-2-oxazolidinone (AOZ) was synthesized at a high yield from the raw materials dimethyl carbonate and ethanol hydrazine hydrate using a catalyst and tetrahydrofuran as a solvent. The results showed that the optimum reaction conditions for maximum AOZ yield (82.7%) were as follows: catalyst dosage, 12%; reaction temperature, 75 ℃; and reaction time, 3 h. Subsequently, a hapten derivative was obtained from the structural transformation of AOZ. Then, immunogens and coating antigens were successfully prepared using a method involving glutaraldehyde to conjugate AOZ and its hapten derivative with activated ovalbumin (cOVA) and bovine serum albumin (cBSA). The hapten and immunogen were identified by gas chromatography-mass spectrometry (GC-MS), elemental analysis, and UV spectrophotometry. The GC-MS data demonstrated the successful preparation of AOZ. The elemental analysis indicated successful formation of the hapten. UV spectrophotometry results showed that conjugation of the hapten with cOVA and cBSA was successful, and the conjugation ratios of immunogen I and immunogen II were 16.74 and 24.05, respectively.
