High Static Pressure and Enzymatic Hydrolysis-assisted Preparation of Super-fine Bone Powder
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Abstract:
To optimize the milling ratio, promote recycling and utilization efficiency of livestock and poultry bones, the effect of high static pressure treatment and enzymatic hydrolysis on milling ratio of porcine bones was evaluated in this study. The process of enzymatic hydrolysis was optimized using response surface technology. The calcium and phosphorus content of milled bone powder was measured. The results showed that the milling ratio was higher when the bones were enzymatically hydrolyzed after high static pressure treatment than prior to treatment. Optimization of the enzymatic hydrolysis process using response surface technology showed that the milling ratio reached a maximum of 82.0% with enzymatic hydrolysis at 55 ℃ for 2.01 h, at pH 7.0 and with enzyme addition of 10,363 U/g. The corresponding milling ratio increased by 9.45% as compared with the non-optimized treatment. Analysis of calcium and phosphorus content in raw bones and milled bone powder indicated that the content of calcium and phosphorus was reduced to a lesser extent by high static pressure and enzymatic hydrolysis-assisted milling. These results suggested that the milling ratio of porcine bone was effectively increased by high static pressure and enzymatic hydrolysis-assisted milling, where the calcium and phosphorus content of milled bone powder were similar to that in raw bone.
