Isolation and Identification of Dibutyl Phthalate-degrading Strain and Its Degradation Characteristics
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Abstract:
In this study, a high-efficiency degrading strain, JF, that utilized dibutyl phthalate (DBP) as the sole source of carbon and energy for growth was isolated from vegetable field soil in Ya’ an, Si Chuan province, where mulch film was used. The strain was identified as Bacillus subtilis based on morphology, physiological and biochemical features, and 16S rDNA sequence analysis. Study on growth dynamics of strain JF in LB medium showed that degradation of dibutyl phthalate conformed to a first-order kinetics equation model. Based on substrate concentration, temperature, and pH, the half-life of DBP was estimated to be between 3.11 and 6.98 h. Additionally, the reaction rate was greatly influenced by substrate concentration and temperature but was not largely affected by pH. The strain was able to degrade DBP as well as its intermediate product phthalic acid. Degradation rate of 200 μg/mL di(2-ethylhexyl) phthalate (DEHP), di-n-octyl phthalate (DOP), and butyl benzyl phthalate (BBP) in 72 h was 90.10%, 79.10% and 76.30%, respectively. This study could provide theoretical basis for the removal of pollutant phthalic acid ester from both, the environment and agricultural products.
