Determination of Penicillin in Honey by the Nano-Ag / Horseradish Peroxidase Self Assembly Immunosensor
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Abstract:
Aminobenzene sulfonic acid, nano-Ag particles and horseradish peroxidase (HRP) labelled penicillin antibody was successfully immobilized on the glassy carbon electrode by the layer-by-layer assembly technique. The poly aminobenzene sulfonic acid/nano-Ag /HRP amplified electrochemical immunosensor was prepared and an enhanced sensitivity electroanalysis method for the detection of penicillin was established. HRP can catalyze the reduction of H2O2 to promote the oxidation of 3,4-diaminobenzonic acid, causing the changes of the peak current, thus realizing the detection of penicillin by the current changes. The effects of buffer solution pH，reaction temperature and time were investigated. Under optimized conditions, the current response of the sensor showed a good linear relationship with the concentration of penicillin within 0.05~6.00 μg/L, with a correlation coefficient of 0.9946 and the detection limit of 1.02 μg/L. The penicillin content in the honey samples was also detected by the modified electrode, and the detection results were compared with the traditional ELISA method. It is proved that this method has good sensitivity and specificity and suitable for detection of penicillin residues in honey samples.
