Effect of NaNO3 on Nostoc flagelliforme Growth under Intermittent Flow of CO2
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Abstract:
In this study, the effect of different concentrations of NaNO3 on the growth, photosynthetic rate, exopolysaccharide accumulation, protein and pigment content, phosphate and nitrate consumption, cell sequestration rate, and photosynthetic efficiency of Nostoc flagelliforme, incubated with intermittent CO2 flow, was investigated. The results showed that addition ofNaNO3with intermittent flow of CO2 could significantly promote the growth of N. flagelliforme. The optimal NaNO3 concentration for biomass production was 2 g/Land the dry weight could reach up to 1.56 g/L, which was 2.29 times as much as that for the control. Maximum respiration as well as net photosynthetic rate was achieved. However, when the concentration of NaNO3 was increased to 2.5 g/L, the biomass no longer increased. Cell chlorophyll content increased with increasing NaNO3 concentration. However, in the NaNO3 group, exopolysaccharide content decreased compared with the groups without NaNO3, and polysaccharide content increased with increasing NaNO3 concentration. The protein content of N. flagelliforme cells increased with increasing NaNO3 concentration and reached maximum value at 2.0 g/L. Corresponding to the increase in biomass, the highest utilization rate of nitrite and phosphate was achieved when the concentration of NaNO3 was 2.0 g/L. With intermittent CO2 flow incubation, addition of NaHCO3 could further promote cellular growth. When two carbon sources co-existed, the growth-promoting effect of NaNO3on cells was similar to that when there was only one carbon source.
