Purification and Structural Analysis of a Polysaccharide from Lentinusedodes Extracted using an Acid Solution
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Abstract:
A new polysaccharide SP2 was isolated from Lentinusedodes using an aqueous HCl solution and purified using gel column chromatography, the purity was determined using filter paper electrophoresis. The relative molecular mass and distribution of SP2 were analyzed by high-performance gel permeation chromatography, and the structure was analyzed by UV-Vis spectroscopy, gas chromatography, infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry, as well as periodate oxidation, Smith degradation, and Congo red experiments. The results indicated that SP2 showed a typical absorption peak for polysaccharides after the color reaction and had a relatively homogeneous composition. In addition, the molecular weight distribution range was relatively narrow, with a weight-average molecular weight of 5.203×104 u. SP2 was found to be composed of mannose, glucose, and galactose with a molar ratio of 0.32:58.6:2.82. Periodate oxidation and Smith degradation experiments indicated that the C-C bonds in the branches of SP2 were composed of (1→2), (1→3), and (1→6) linkages with a molar ratio of 9.43:5.44:1. SP2 was found to be a glycoprotein conjugate containing pyranose rings that was amorphous and could not form the monocry stalline structure. SP2 contained spiral structures and had no strong intermolecular interactions. These results provided evidence to explain the structure-activity relationships of polysaccharides from L. edodes.
