PCR-DGGE to Detect Bacterial Flora Change in Liquid-smoked Tilapia Fillets during Storage
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Abstract:
Denaturing gradient gel electrophoresis technique based on polymerase chain reaction (PCR-DGGE) was used to investigate the constitution and changes of bacterial flora in the liquid-smoked tilapia fillets during storage, in order to provide a theoretical foundation for their storage conditions. The results showed that 13 bands were isolated using DGGE and after analysis their sequences, 10 of them had sequence similarity above 90%. These included two strains of Enterobacter, two strains of Clostridium, two strains of Lactococcus, two strains of Pediococcuspentosaceus, one strain of Leuconostoc, and one strain of Pseudomonas. Lactic acid bacteria (LAB) accounted for 50% of all bacteria. The bacterial flora during early storage (<2 d) and the last storage period (>7 d) were quite complex, with more than 10 identified bands. During the mid-storage period (2~7 d), however, the bacterial DGGE bands were distinctly grouped and there were only approximately five identified bands, indicating that the microbial diversity decreased. The bands from DGGE and sequencing results indicated that the dominant bacteria during storage were Lactococcus, Pediococcuspentosaceusand Clostridium.
