Anti-tumor Effect of an Acid Polysaccharide from Gracilaria lemaneiformis in H22-bearing Mice
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In this study, the inhibitory effect of an acid polysaccharide from Gracilaria lemaneiformis (GLSPs) on H22-bearing mice was investigated. The influence of GLSPs on the cell cycle and apoptosis of tumor cells in vivo was studied using flow cytometry. The effect of GLSPs on splenetic lymphocyte proliferation and killing activity of NK cells in tumor-bearing mice were determined by MTT assay. The tissue pathological detection of the immune organs (spleen and thymus) in mice was performed. The results showed that apoptosis of H22 hepatoma cells in mice was promoted by GLSPs, and tumor growth was remarkably inhibited. Intragastric administration of GLSPs at 200 mg/(kg·d) and 600 mg/(kg·d) in tumor-bearing mice for 28 days resulted in an average inhibition rate of tumor volume of 84.65% and 94.58%, respectively. Immunological tests in tumor-bearing mice showed that pathological changes, including splenic enlargement and thymic atrophy, were significantly improved, and the function of the immune organs was reinforced. Moreover, the drastic increase of leukocytes and lymphocytes in the blood of tumor-bearing mice was effectively regulated, while the proliferation of T and B cells induced by ConA and LPS as well as the killing activity of NK cells were significantly improved. Thus, the immunity of tumor-bearing mice was enhanced through different mechanisms. These results indicate that GLSPs have anti-tumor effects in vivo via immunomodulation.
