Determination of Naproxen in Health Liquor Using HPLC-PAD
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Abstract:
Recently, undeclared anti-inflammatory drug were frequently exposed being adulterated into antirheumatic health liquors to promote therapeutic effect. This phenomenon poses a serious threat to public health. Naproxen is a common nonsteroidal anti-inflammatory drug. The aim of this study is to develop a liquid chromatographic method coupled with photodiode array detector for identification of illegal adulterated naproxen in healthy food. Two-step liquid-liquid extractions were employed to purify naproxen from samples using chloroform in acidic condition following aqueous solution in alkaline condition, respectively. Chromatographic separation was achieved on a Kromasil 100-5C18 column (250×4.6 mm E74572) with the mobile phase of methanol-10mM phosphate buffer (pH 4.0) (75/25, V/V), at a ?ow rate of 1.0 mL/min. The eluant was detected at 231nm. Retention time combined with standard addition result and PAD-scanning graph were applied to verify naproxen. Quantitation of naproxen was attempted by external standard method. The results showed as follows: the limit of quantitation was 0.5 μg /mL, the linear range was 5~80 μg/mL (R2= 0.9993), RSD was 1.87% (n=6) and the recovery of naproxen from health liquor was ranging from 83.3~92.6%. The method is suitable in determination of naproxen as adulterants in health liquor due to its accuracy and reliability.
