The Research of Apoptotic in Yak Muscles during Postmortem Aging
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Abstract:
Twenty-four Gannan yak were slaughtered according to Islamic way of slaughtering, then aged at 0~4 ℃ with wind speed of 0.5 m/s . The shear force, MFI, color, apoptotic rate and caspase-3 activity during postmortem aging were determined. The shear force value in triceps brachi (TB), musculus longissimus (ML) and semimembranosus (SM) after slaughtered of 1~5 d was significantly lower (p <0.05), and MFI significantly increased (p <0.01); L *, a *, b * values were significantly higher at the 3rd d of postmortem aging, a *, b * values decreased significantly at 5~7th d of postmortem aging (p <0.01); the cooking loss first increased and then decreased at 1~7th d of postmortem aging; the apoptosis rate in TB and SM had very significant increase in process (p <0.01), the positive nuclei number in TB and ML had difference during postmortem aging (p <0.01); it was detected caspase-3 activity in TB, ML and SM at 0.5 h postmortem, while it was reached the highest in ML at 12 h postmortem. Across correlation analysis, the yak skeletal muscle cell apoptosis and its degree of deepening were related to the changes of tenderness, meat color (L *, a *, b *) and water holding capacity, as well as the yak meat quality change.
