Differentiation of Food-borne Fatty Acid Biomarkers in Hybrid Abalone Haliotis discus hannai and Haliotis discus discus
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Abstract:
To determine suitable tissue in hybrid abalone used for long-term food sources, distribution characteristics of food-borne fatty acid biomarkers in digestive gland, muscle and visceral mass of bottom-sowed hybrid abalone were investigated, which were useful to differentiate long-term food sources of hybrid abalone. There were no significant differences in diatom fatty acid biomarkers (∑C16/∑C18 and C20:5n-3) and heterotrophic bacteria fatty acid biomarkers (C18:1n-7/C18:1n-9) among visceral mass, digestive gland and muscle of hybrid abalone with similar body size. The Chlorophyta fatty acid biomarkers (C18:2n-6+C18:3n-3) in digestive gland was higher than that in muscle (p<5 %), while Phaeophyta fatty acid biomarker (C20:4n-6) had the opposite result to Chlorophyta. Fatty acid compositions in visceral mass were affected by the development of gonad; fatty acid content in muscle was low. Total fatty acid content in digestive gland was the highest, and the fatty acid composition varied with the seasonal differentiation of food sources. From points of factors influencing composition of fatty acids and measurement sensitivity, it was better to determine digestive gland as long-term food sources of abalone by fatty acid biomarkers analysis.