Application of Loop-mediated Isothermal Amplification on Pathogens Rapid Detection Key words:
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Abstract:
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method with many fragment amplification products according to specific region of target genes. Currently, precipitation, electrophoresis and fluorescence are the main methods to detect amplification products. Compared with PCR, amplification and detection process can be achieved in one step without template thermal denaturation, long time cycle and complex electrophoresis. Therefore, LAMP has been reported and applied in the field of bacteriological detection with advantages of rapid, easy operating, low-cost, highly sensitive and specific detection. In future, it is convinced that LAMP will replace PCR assays, contributing to more rapid, simple, specific and accurate detection method. Nowadays, LAMP has been applied on detection of various pathogenic microbes and embryo sexing, and the microbes include pathogenic bacterial, fungi, virus and parasites. This detection has been employed in different fields, such as food safety, clinical medicine and fishery. This paper described the features of LAMP and its application on rapid detection of bacterial, viruses, parasites and aquatic animal pathogens.
