The Contamination Investigation and ERIC-PCR Typing of Yesinia enterocolitica in Foods
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Abstract:
The contamination of Yesinia enterocolitica in seven kinds of food from 17 cities across the country was investigated for Y. enterocolitica distribution and genotypic diversity analysis. The microbiological examination of food hygiene--examination of Y. enterocolitica (GB/T4789.8-2008) and GB/T4789.8-2003 were slightly modified and used as the Y. enterocolitica detection methods. The ERIC?PCR fingerprinting of Y. enterocolitica was carried to typing. Fifty positive samples of Y. enterocolitica were detected from 946 food samples, and the total Y. enterocolitica contamination rate was 5.29%. Among the 50 positive samples, the raw meat, frozen food and edible fungi were 24 (12.06%), 25 (16.89%) and 1 (0.74%) respectively. The positive samples detected by GB/T4789.8-2003 method were 34 (3.59%) while were 25 (2.64%) by GB/T4789.8-2008. Cluster analysis showed that 72 Y. enterocolitica strains from 50 samples were divided into four groups and the type C was the main genotype, which initially established the Y. enterocolitica ERIC-PCR fingerprinting database .
