A Method for Fast Quantitative Analysis of Major Wine Volatile Compounds Based on Liquid-liquid Extraction
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Abstract:
In this paper, 3 mL wine was diluted with water (7 mL) and internal standard solution (60 μL 0.02% (V/V) 4-Methyl-2-pentanol), salted with 4.5 g ammonium sulfate and then extracted with 0.5 mL dichloromethane. After scrolled for 10 min and centrifuged for 10 min, the extract was injected in a GC system. ZB-WAX capillary column (30 m × 0.25 mm × 0.25 μm) used with the temperature program was as follows: 40 ℃ for 5 min, then raised at 3 ℃/min up to 190 ℃ for 10 min. N2 was used as carrier gas with flow rate of 1.5 mL/min, injection volume and input temperature were 0.5 mL and 200 ℃, and the injection mode was splitless. The results were detected by FID. The method could isolate 63 compounds from wine, and 30 of them were analyzed qualitatively. They included nine alcohols, eleven esters, eight organic acids, two aldoketones. Repeatability of 28 of them was less than 10% (as RSD) with the recoveries from 85% to 120%. The quantitative analysis by using internal standard solution was accurate. This method has the advantages of simple operation, little pollution, short time of pretreatment and good repeatability and accuracy, which satisfies the further demand of wine fingerprinting analysis technology.
