Development of a Colloidal Gold Labeled Strip for the Rapid Detection of Bisphenol A
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
To establish a rapid method for detection of Bisphenol A (BPA), colloidal gold-based immune chromatographic assay (GICA) was used. After using artificial antigen of BPA to immune Balb/C mice, monoclonal antibodies against BPA were prepared by normal hybridoma technology. The monoclonal antibody was purified by precipitation of Caprylic Acid-Ammonium Sulfate (CA-AS) and it was identified as IgG2b subtypes. Colloidal gold was prepared through the reduction of gold chloride by sodium citrate acid, UV-Vis spectrum and transmission electron microscope (TEM) were performed, the results indicated that the colloidal gold had a single 530 nm absorption peak and its particle size was about 30 nm. The colloidal gold-antibody was produced by monoclonal antibody of BPA labeled with colloidal gold. In this study, the GICA sensitivity was about 100 ng/mL and the detection time was about 10 min. The cross-reactions showed that there was no cross-reaction between test strip and BPA analogues, but had good reproducibility. This method was convenient, rapid and effective for detecting BPA, and the development of GICA was applicable for the rapid determination of BPA in the field.
