Comparison of Different Pretreatments for GC Determination of L-Borneol, Isoborneol, and Camphor Contents in Blumea balsamifera (L.) DC. Leaves
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Abstract:
The efficiencies of hydrodistillation-solvent extraction (HDSE), simultaneous distillation and extraction (SDE), solvent extraction (SE), ultrasonic assisted extraction (UAE), microwave assisted extraction (MAE), and simultaneous ultrasonic-microwave assisted extraction (UMAE) in the extraction of L-borneol, isoborneol, and camphor from leaves of Blumea balsamifera (L.) DC. were compared. Three compounds in leaves were determined by GC with six pretreatments, respectively. UMAE was the most rapid pretreatment method (extraction time 30 s). The precisions of six pretreatments all met the standard, with RSDs of less than 3%. GC combined with six pretreating was accurate in the determination of L-borneol (recoveries 86%~100%). However, HDSE-GC was the only method for detecting all three compounds (recoveries 100%~105%). The leaves of B. balsamifera from harvest period were determined by HDSE-GC and UMAE-GC, and it was found that L-borneol contents in leaves harvested from mid-October to next mid-January were high, suitable for raw material in L-borneol production. The study provides an accurate and reliable pretreatment method for the rapid analysis of L-borneol in B. balsamifera leaves and the best harvest period of leaves.
