Fatty Acids and Stable Carbon Isotope Ratios in Cordyceps militaris
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Abstract:
This paper aims to study the fatty acid composition and stable carbon isotope ratios as well as their variational trends of several substitutes of Ophiocordyceps sinensis. Firstly, the stable carbon isotope ratios of eight substitutes of Ophiocordyceps sinensis, including six kinds of Cordyceps militaris and two kinds of Cordyceps mycelium, and one stroma sample of Ophiocordyceps sinensis were determined by elemental analysis-isotope ratio mass spectrometry (EA-IRMS). Then, the neutral and polar lipids were effectively extracted from the samples by a chemical saparation method, and their fatty acid compositions were measured by chromatography mass spectrometry (GC-MS). Finally, the measured data was compared with our previously-published data of Ophiocordyceps sinensis. Evident differences were found among different substitutes of Ophiocordyceps sinensis in their stable carbon isotope ratios, fat contents and fatty acid compositions. The contents of fat and unsaturated fatty acids in the substitutes were lower than those of Ophiocordyceps sinensis, exhibiting the irreplaceability of Ophiocordyceps sinensis. Results also showed that the stable carbon isotope ratios and fatty acid composition might be two important markers to discriminate the adulteration among the substitutes of Ophiocordyceps.
