Isolation, Determination and Antibiotic Resistant of Food-born Providencia spp.
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Abstract:
The objective of this study was to identify the Providencia spp. that isolated from three kinds of different meat and their antibiotic resistance performance was also investigated. API20E system was used to determine and identify the isolates from pork, chicken and beef. And Kirby-Bauer was applied to detect the antibiotic resistance of Providencia spp. Subsequently, Providencia spp. was screened by PCR for class ? integron. The results showed that 38 samples were Providencia spp. positive in 85 samples, and the relevance ratio was 44.70%. Moreover, 44.74% of positive Providencia spp. was multiple resistant and some of them resisted up to six kinds of antibiotics. Two isolates showed class ? integrase positive, and one isolate of Providencia rustigianii was found carrying gene cassette. These isolates with multiple resistances in the food may be transferred to human by food chains, and then become a potential threat for human health.
