Antitumor Activity of Glycosaminoglycan from Crassostrea rivularis
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Abstract:
The anti-tumor activity of glycosaminoglycan from Crassostrea rivularis (CG) in vivo and in vitro was studied in this paper. MTT method was used to detect the effect of CG on the growth of K562, CNE-2Z and Hela cells in vitro. Through the method of animal transplant tumor screening in vivo, the effects of CG on the growth of S180, EAC and L 1210 tumors in mice were observed. The results showed that the fractions of CG, CGIa and CGIIa could inhibit the growth of K562, CNE-2Z and Hela cells (p<0.05), and when combined with 5-Fu the efficiency of anti-tumor increased. IC50 of CGIa on K562 and CNE-2Z cells in 72h were 1.95 mg/L and 7.36 mg/L respectively. In vivo, CG had remarkable inhibitory effect on growth of S180 tumor. The inhibitory ratio increased from 44.66% to 67.96% when combination with CTX. The immune injury by CTX could be repaired by CG. The life prolonging rate of mice transplanted with EAC and L1210 tumor while treated by CG combination with CTX were 50.27% and 55.27%. The results showed that glycosaminoglycan from Crassostrea rivularis had anti-tumor activity.
