Comparative Study of Extraction Conditions and GC-MS Analysis of Hydrocarbons from Botryococcus sudeticus
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Abstract:
Based on ANOVA analysis of the solvents, temperatures and time, the optimal conditions for hydrocarbons extraction from small amount of algal powder of Botryococcus sudeticus were obtained as follows: 40 mg algal powder suspended in n-hexane was ultrasonicated at 35 ℃ and 70 Hz for 30 min, then extracted at 35 ℃ for 10 hours. The highest yield of hydrocarbons accounted for 2.95 ‰ of dry weight was obtained under above conditions. The programmed procedure for GC-MS analysis was compared and optimized, and long-chain alkanes from C22 to C31 were found as the dominant hydrocarbons in the lyophilized powder of Botryococcus sudeticus from the cells in exponential phase. They were consisted of docosane, tetracosane, pentacosane, 9-octylheptadecane, heptacosane, octacosane, squalene and hentriacontane, with contents of 50.40, 209.17, 351.45, 472.38, 540.48, 542.24, 473.60 and 306.86 μg/g of dry weight, respectively. This extraction and analysis process have advantages of fewer amounts of sample required, time-saving, high yield and simple operation, which is a rapid and high efficient method for the analysis and detection of hydrocarbons from Botryococcus sudeticus.
