Fermentation Optimization of a Novel Recombinant Peroxiredoxin in Pichia pastoris for Zearalenone Detoxification
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Abstract:
In order to maximize the expression of a novel peroxiredoxin purified from Acinetobacter sp. SM04 in recombinant Pichia pastoris GS115/pPIC9K-A4-Prx, a single-factor test coupled with response surface methodology (RSM) was used to adjust the induction condition. The results showed that pH value, temperature and glycine concentration were significant influencing factors for A4-Prx expression, and the optimized induction conditions were as follows: pH 7, 0.11% glycine at 30 ℃, and the theoretic yield of A4-Prx could reach to 129.873 mg/L. Then further experiments under the optimized induction conditions illustrated that the concentration of the novel peroxiredoxin synthesized by Pichia pastoris GS115/pPIC9K-A4-Prx was 128.941 mg/L and had a significantly ZEA-degrading effect on DDGS and some food materials. This study laid the foundation for the high-density industrial fermentation of peroxiredoxin as well as the development of ZEA degradation.
