High Cell Density Fermentation of Lactobacillus salivarius XH4B Via pH Adjustment
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Abstract:
Culture medium ingredients of nitrogen, carbohydrate and inorganic salts were optimized based on MRS for high cell density fermentation of Lactobacillus salivarius XH4B, including the adjustment of pH value as well as culture medium fed-batch. According to the biomass of L. salivarius XH4B, the best nitrogen and carbon sources were yeast extract and sucrose, respectively. Addition of sodium acetate (2%) and monosodium orthophosphate (0.6%) provided buffer effect for the pH value during incubation, these salts also contributed to LAB growth. By using the optimized PY-Suc culture medium, the biomass of L. salivarius XH4B reached 6.36~6.91 g/L, higher than that in MRS culture medium which was 5.01~6.30 g/L (P < 0.05). When lactic acid concentration exceeded 10 g/L, the accumulation of biomass ceased which meant the acid inhibited L. salivarius XH4B growth. Therefore, the optimization of MRS culture medium with sodium acetate and monosodium orthophosphate could buffer the pH value of culture medium, and new culture medium combined with fed-batch fermentation resulted in high cell density of L. salivarius XH4B.
