Comparison of Physical and Chemical Characteristics of Collagen from Silver Carp Skin using Different Extraction Methods
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Abstract:
Acid soluble collagen (ASC), pepsin soluble collagen (PSC) and hot water-soluble collagen (HWSC) were successfully extracted from silver carp skin, and the physical and chemical characteristics of them were investigated and compared. The results showed that UV and FT-IR measurements of ASC, PSC and HWSC were quite similar, and all of them showed maximum absorption peak at 232 nm and almost no absorption peak at 280 nm. SDS-PAGE pattern indicated that all the collagen extracted by three methods contained two different α chains -(α1) 2α2, and their amino acid composition and proportion were similar. It was conformed to the characteristics of type I collagen. The hydroxyproline (Hyp) content of HWSC was lower than those of other two extracting methods, but the gel property by this method was better. The acid extraction had minimal effect on the network structure of collagen. The thermal denaturation temperature of ASC, PSC and HWSC were 31.05±0.14 ℃, 31.45±0.01 ℃ and 43.75±1.20 ℃, respectively. The Hyp content, rheological characteristics, thermal denaturation temperature and the microstructure of collagen were affected by different extraction methods.
