Screening of Lactic acid bacteria for Production of Anti-listeria Bacteriocin
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Abstract:
By double agar diffusion methods, the strain producing bacteriocin with inhibitory activity against Listeria monocytogenes was screened and identified from Chinese northeast fermented cucumber. And the bacteriocin was separated and purified and its molecular mass and stability were researched. After eliminating the effects of organic acids and hydrogen peroxide, the strain of Lactobacillus spp. ZLG85 which still had activity against Listeria monocytogenes was screened. The inhibitory activity was degraded sharply by pepsin, trypsin and neutral protease in enzymatic hydrolysis, which was confirmed that ZLG85 can produce bacteriocin. By appearance detection, physiological and biochemical characteristics analysis and 16S rDNA gene sequence homology analysis, ZLG85 was identified as Leuconstoc mesenteroides subsp.mesenteroides. Physical and chemical analysis showed that the mesentericin ZLG85 was heat-stable. Its relative inhibitory activity was 93.77% after incubation at 121 ℃ for 30 min, and above 80% after incubation 4 h within a wide pH range from 2.0 to 10.0. The molecular weight of mesentericin ZLG85 was 2.5 kDa conformed by tricine-SDS-PAGE electrophoresis. Mesentericin ZLG85 is a new kind of bacteriocin with strong stability against Listeria monocytogenes.
