Separation and Antioxidant Activity Evaluation of Acaudina molpadioides Peptide
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Abstract:
Amino acid composition of body wall powder and hydrolytic peptide from Acaudina molpadioides (A. molpadioides) was analyzed and in vitro antioxidant activities of peptide separated from the hydrolytic fractionations were detected. The total oxidant activity of A. molpadioides hydrolytic peptide was evaluated. Results showed the both body wall and hydrolytic peptide contained 19 kinds of amino acids, which were occupied 63.06% and 82.90% of sample dry weight, respectively. Among which, the essential amino acid content were 10.87% and 15.60%, respectively. Three compositions with different molecular weight were separated from hydrolytic peptide by ultrafiltration. The small peptides (<3 kDa) took over the highest level (43.9%) , followed by 3k-10kDa molecular weight peptides(36.8%), and the polypeptide above 10kDa was the minimum (19.3%). The ABTS and FRAP assays showed that compared to other compositions, the antioxidant activity of small peptides (<3 kDa) was the highest, the TEAC value was 0.61 ± 0.03 mmol Trolox/g and 0.32 ± 0.02 mmol Trolox/g, respectively. The result of cell culture experiments displayed that the three compositions from A. molpadioides hydrolytic peptide significantly enhanced the ability of HEK293 and HepG-2 cells against H2O2 oxidative damage. The small peptides (<3 kDa) appeared to be more active than the other components. Six major peaks in the small peptide composition were observed by glucose gel separation with G-25, and the highest antioxidant activity peptide was detected in the sample of the 5th fraction.
