Screening and Identification of Amine Oxidase Productive Strains from Smoked Horse Intestine
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Abstract:
In order to obtain good amine oxidase productive starters for meat product, amine oxidase productive strains were isolated from 44 samples in different areas of the smoked horse intestine. Productive amine bacteria were eliminated by double color method in monolayer culture. The oxidase test, bacteriostasis experiment and the resistance to salt and nitrite test were conducted for isolated strains. The same strains were eliminated by DGGE and the ability of oxidizing biogenic amine was verified by HPLC. Finally, the DNA was extracted from the screened strains, and V6-V8 segment of 16s rDNA were sequenced after PCR amplification. The results showed that six strains productive amine oxidase bacteria were screened out, with salt tolerance above 6%, nitrite tolerance above 0.15 g/kg, and had good inhibitory effect to Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus, and they had strong oxidizing ability of biogenic amines. The strains were Lactobacillus rhamnosus, Bacillus subtilis, Staphylococcus saprophyticus, Staphylococcus xylosus, Pediococcus pentosaceu and Lactobacillus plantarums. This research provides a good theoretical basis for the industrial production and security of fermented meat.
