Analysis of Antioxidant Activities of Oligopeptides from Sea Cucumber Guts Based on ESR and Cell Culture System
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Abstract:
In this paper, the antioxidant activities of oligopeptides (Val-Gly-Thr-Val-Glu-Met and Val-Thr-Pro-Tyr) from sea cucumber guts were studied by measuring the scavenging abilities on hydroxyl radical, superoxide radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical using an ESR spectrometer. Moreover, the influence of oligopeptides on H2O2-induced oxidative damage in Jurkat cells was also investigated. The results showed that two kinds of oligopeptides from sea cucumber guts showed scavenging abilities on ?OH, O2-? and DPPH? in a dose-dependent manner. The scavenging ability of Val-Gly-Thr-Val-Glu-Met was significantly higher than that of Val-Thr-Pro-Tyr at different concentrations (p<0.05). The oligopeptides failed to protect the DNA oxidative damage induced by H2O2 at concentrations of 5 mM in Jurkat cells, while induced cell death at concentration of 20 mM. These results suggest that the oligopeptides from sea cucumber guts (Val-Gly-Thr- Val- Glu-Met,Val-Thr-Pro-Tyr) possess free radical scavenging abilities, and have certain development value.
