Extraction of Anti-tumor Constituents Derived from Dendrobium officinale
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Abstract:
To explore the anti-tumor constituents from Dendrobium officinale, the components extracted with three solvents (ethyl acetate, 95% ethanol and water) were separated and purified by silica gel column chromatography, TLC and Sephadex LH-20, and the whole process was guided by MTT method. Results showed that the strongest activity component named TD1 was achieved by the ethyl acetate extraction, the proliferation of human hapetocelluar carcinoma cell line (HepG-2), human gastric carcinoma cell line (SGC 7901) and human breast cancer cell line (MCF-7 ) were significantly inhibited by TD1 and the IC50were 0.45 μg/mL, 0.36 μg/mL and 0.34 μg/mL, respectively. TD1 was identified as denbinobin by spectroscopic data. Denbinobin was first isolated from Dendrobium nobile Lindl. and its anti-tumor effect has been certified and widely noted in recent years, the results may provide references for the development of new medicine and health food by using Dendrobium officinale as raw materials.
