Influence of Acylation on the Structures and Physico-chemical Properties of Yak Caseins
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Abstract:
The influence of different acylation reagents (succinic anhydride, succinic acid and acetic anhydride) on the spatial conformation and physico-chemical properties of yak caseins were investigated. The secondary and tertiary structure, buffer capacity and calcium, magnesium, phosphorus content of original and modified yak caseins were also studied. The results showed that the acylation degree with succinic anhydride, acetic anhydride and succinic acid was 90.0%, 87.9% and 18.5%, respectively. After acylation with different reagents, the secondary structures of yak casein were mainly turn and β-sheet; a few α-helix structures formed after succinylated modification, with the content of 9.4%. Succinic anhydride had the largest influence on tertiary structure of casein, followed by acetic anhydride and succinic acid. The maximum ?uorescence intensity of intrinsic and ANS ?uorescence spectrum were reduced with modification and the maximum emission wavelength were shifted red except acetic anhydride modified casein. The modification improved the buffer capacity, reduced the content of calcium, magnesium, phosphorus. The color of modified caseins was whiter than original casein. The results could provide references for the chemical modification of yak caseins.
