Establishment of a Liquidchip Method for Listeria Monocytogenes Detection
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Listeria monocytogenes liquidchip detection method was established by coupling Listeria monocytogenes monoclonal antibody (mAb930) and polystyrene microspheres, and combining with double antibody sandwich technique. The ratios of different concentration of antibody to the microsphere coupling were detected. L25(56) orthogonal test was used to optimize the reaction conditions, and verification of the sensitivity and specificity were detected by the developed method. 200 food samples were tested by the method, with comparison of the national standard method. The optimal concentrations of polyclonal antibody, , the antibody biotin labeled Goat anti rabbit IgG and SA-PE antibody were 1:100, 1:500 and 10 μg/mL, respectively. And the best reaction time of biotin labeled and SA-PE was, 30 min. The sensitivity of this method reached 103 CFU/mL. No cross reaction with other common food borne pathogenic bacteria occurred. In addition, the results were consistent with the national standard method. The false positive rate of liquid chip assay for the detection of various samples was less than 0.77%. The method has high sensitivity, strong specificity, good reproducibility, and can be used for rapid detection of Listeria monocytogenes in food.
