Spectrophotometric Determination of Taurine in Shellfish by Inhibiting Methyl Orange Oxidation
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Abstract:
Trace taurine has obvious inhibitory effect on the reaction of potassium hypermanganate oxidizing methyl orange in ammonium chloride-ammonia buffer solution at pH 11.0, resulting in fading of the system and great decrease of absorbance. The acidity, potassium hypermanganate and methyl orange dosage, temperature and time were optimized. Under the optimum reaction conditions, good linear relationship was obtained between the decreased value of absorbance and taurine concentration in the range of 0~75 μg/L with the maximum fading wavelength of 690 nm. The apparent molar absorption coefficient was 2.22×106 L/(mol?cm), with detection limit of 1.19 μg/L. Samples were pretreated using hot water extraction and ion exchange , the proposed method was applied to the determination of taurine in Meretrix meretrix, Sinonovacula constricta, Chlamys farreri and Mactra veneriformis. The results agreed well with those obtained by high performance liquid chromatography. The relative standard deviation was 0.82~1.24% (n = 6), and recovery was 98.31~101.76%. This method was sensitive, accurate, simple, rapid and environmentally friendly under, which was suitable for determination of taurine in shellfish.
