Establishment of Growth Model of Staphylococcus aureus Based on OD-TTD
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The growth model of Staphylococcus aureus CMCC (B) 26003 based on growth rates and lag time calculated by OD-TTD data was studied in this paper. The culture was serial diluted and static cultivated in 96 pores micro-array plate at 37 ℃. When the linear relationship between the initial inoculums (N0) and the time to detection criterion of OD at specific value (TTD) was available, the growth rate could be calculated. If the viable count from well with OD at detection value (Nd) was known, the lag time could also be obtained. Then a suitable model was selected to fit the growth data. The linear relationship between TTD and N0 as follows: TTD=-154.86 N0+1202.3, R2 = 0.9997 (OD = 0.1). The growth rate of 0.0064 log [cfu/(mL?min)] (μ) and lag time of 7.8 min (λ) were obtained by the formula. If the information of μ,λ,N0, Nmax and Nd were available, and TTD could be calculated by Gompertz, Baranyi, Logistic and 3-phase linear (3PLM) model. In addition, the calculated value by 3PLM was congruent with the observed value. The growth rate from OD-TTD was almost equal with that from viable plate count, and the lag time from viable plate count was bigger than that from OD-TTD. Therefore, 3PLM based on OD-TTD was suitable to construct the growth model of Staphylococcus aureus, but the lag time from OD-TTD was uncertain yet.
