Inhibitory Effect of Rubus corchorifolius Leaves Extract on the Movement of Isolated Intestinal from Rabbit and Its Active Stability
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Using the movement of isolated intestinal from rabbit as the model, the extraction of anti-diarrhea active components from Rubus corchorifolius leaves and their active stability were investigated. The active components were extracted with 80% ethanol, followed by five different polar solvents. The effects of temperature, pH, light quality, illumination time, and the tannins removal treatment on the activity of the extracts were also studied. The results showed that the active components mainly existed in ethyl acetate layer, the sequence of the extract activity in different solvent layer was petroleum ether layer>water layer>n-butanol layer>chloroform layer. The ethanol extract of Rubus corchorifolius leaves were stable in the range of 30~60 ℃. But at high temperature of 90 ℃ and 120 ℃, the tensional variation percentage of isolated intestinal from rabbit decreased by 15.43% and 32.45%, respectively. pH showed significant influence on the activity, and the highest activity was found under acidic conditions, followed by neutral and alkaline conditions. The tensional variation percentage was only 11.90% at pH 12. The ethanol extract after different light qualities and different illumination time treatments still had high activity (P<0.01), while the activity was decreased by tannin removal treatment, indicating tannin might be one of the components for anti-diarrheal activity in Rubus corchorifolius leaves.
