In vitro Evaluation of Immunomodulatory Activity for Polysaccharide LPII from Arillus longanae
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Abstract:
LPII is one of the main fractions of polysaccharides from Arillus longanae. The immunomodulatory activities of LPII, and the effect of enzymolysis on its activity were investigated in vitro. Splenic lymphocytes were injected into a nylon fiber column to separate T cells and B cells. The stimulating effects of LPII on lymphocytes in the dose range of 25~400 μg/mL indicated that LPII could stimulate the proliferations of splenic lymphocyte and B cell and promote the antibody production of B cell. However, its activation on T cells was not significant. In addition, LPII could effectively enhance the proliferation, phagocytosis and NO production of macrophage in the dose range. The fraction of LPII with higher molecule weight obviously degraded by papain, pronase or trypsin, following with the weakened effect on ConA-induced splenic lymphocyte proliferation (P < 0.05) and the strengthened effects on LPS-induced splenic lymphocyte proliferation and macrophage phagocytosis (P < 0.05). LPII has the significant immunomodulatory activity in vitro, and the activity can be enhanced with appropriate degradation, which is confirmed as the most important functional component in Arillus longanae.
