Structure and Antitumor Activity of Water-soluble Polysaccharide from Craterellus cornucopioides
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Abstract:
One polysaccharide, named CCPⅠ, was isolated and purified from the sporophore of Craterellus cornucopioides. The relative molecular mass and monosaccharide composition of CCPⅠ were determined by using high-performance gel permeation chromatography and gas chromatography, and its spectra of IR and NMR were also investigated. The weight-average relative molecular mass of CCPⅠ was 16270 Da. It possessed typical infrared absorptions of polysaccharides and showed α- and β-configuration pyranoside. In addition. It was determined as a kind of heteropolysaccharide containing fucose, mannose, glucose and galactose in a molar ratio of 1:2.11:3.13:6.45. Most of glycan C-6 were substituted, and part of C-2, C-3 or C-4 was replaced. Moreover, its anti-complement activity in vitro and antitumor activity by MTT were evaluated, which indicated that the anti-complement activity reached 70.44% when the concentration of CCPⅠ was 4 mg/mL and it remarkably inhibited the growth of human liver cancer cell line Hep G2 and human breast cancer cell line MCF-7 in a dose-dependent manner. When the concentration of CCPⅠ was 1000 μg/mL, the inhibition ratios of Hep G2 and MCF-7 were 60.06% and 55.80%, respectively.
