Fingerprint of the Litchi Honey by Raman Spectroscopic
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Abstract:
Laser Raman spectrum technology under 785 nm was used to establish the litchi honey fingerprints. The Roman spectra of litchi honey showed that several characteristic strong peaks were found at 424, 521, 629, 706, 820, 867, 918, 1079, 1122, 1266 and 1460 cm-1. And a few obvious Raman peaks appeared at 452, 596, 776, 918, 981 and 1337 cm-1. The Raman peaks showed spectral features of amide, amino acids, proteins, carbohydrates, etc. It was basically confirmed that litchi honey contained protein, amino acid and carbohydrate, which was basic consistent with the known litchi honey biochemical components. Meanwhile, Matlab fingerprint analysis software was used for similarity analysis, it was demonstrated that the similarity of different batches of litchi honey was no less than 0.97 indicating the litchi honey fingerprint had characteristic. There were significant changes before and after processing litchi honey in peak shapes and peak intensities through analysis of first and second order derivatives. Thus, the main ingredients in litchi honey can be directly identified by Roman spectra and the established fingerprint Raman spectroscopy can provide certain theoretical basis for the identification and quality control of lichi honey.
