Detection of Saccharomyces cerevisiae and Listeria monoeytogenes by Flow Cytometry
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Abstract:
The viable and heat-treated microorganisms such as Listeria monocytogenes and Saccharomyces cerevisiae were investigated in this study. They were stained by fluorescent staining reagents SYTO-9 and Propidium Iodide (PI) and then the red and green fluorescence signals were detected using flow cytometry to get the concentration of cells in samples. The results showed that flow cytometry was able to detect bacteria and yeast after the nucleic acid fluorescent staining. This method simplified the procedure for L. monocytogenes and S. cerevisiae detection, shortened the time of detection procedures, and also distinguished viable with heat-treated micrograms and viable bacteria with yeast in the same system. This method was capable of detecting as few as 1.2×104 cells/mL L. monocytogenes and 6 × 103 cells/mL S. cerevisiae. This improvement might shorten the time consuming of culture enrichment or simplify the process.
