Screening and Antifungal Mechanism of Chinese Herb Extracts against Green Mold of Citrus
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Abstract:
The antifungal activity of some Chinese herb extracts on citrus green mold (Penicillium Digitatum) were screened by Cylinder-plate method, the potential antifungal mechanism of the optimum extracts were investigated by determining the changes of electrical conductivity of culture media. The results showed that the ethanol extracts of Cinnamomi Ramulus, Cinnamomi Cortex and Mosla Chinensis had better inhibition effect on the green mold, the inhibition zones were all over 20 mm. Moreover, Cinnamomi Ramulus displayed remarkable inhibition effect, which was even better than Bellkute. The inhibition zone, minimum inhibitory concentration (MIC) and minimum fungicidal concentrations (MFC) were 31.56 ± 0.66 mm, 6.25 mg/mL and 12.5 mg/mL, respectively. In addition, the research also showed that Cinnamomi Ramulus extracts effectively inhibited spore germination and mycelial growth and development. When the concentration of Cinnamomi Ramulus extracts was 50 mg/mL, the conidia germination inhibition rate were up to 99.007%; the permeability of cell plasma membrane and cellular components exosmose were increased; the total soluble protein and sugar contents of mycelium were 1187.18 μg/g and 0.307%, respectively; and the morphology of mycelium were malformed and fractured. Thus, Cinnamomi Ramulus ethanol extracts have good antifungal activity through disturbing the structure of cellular membrane and mycelium metabolism.
