Inhibitory Effects of Chlorogenic Acid and Isochlorogenic Acid from Purple Sweet Potato Leaves on α-glucosidase
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Chlorogenic acid (1), 4,5-O-caffeoylquinic acid (2), 3,5-O-caffeoylquinic acid (3) and 3,4-O-caffeoylquinic acid (4) were isolated and prepared from purple sweet potato leaves by high speed counter-current chromatography (HSCCC). Then the inhibitory effect of these compounds and crude extract of sweet potato leaves on α-glucosidase were examined by using PNPG as substrate. The results indicated that four compounds and crude extract of sweet potato leaves exhibited certain inhibitory effects on α-glucosidase, with IC50 value of 7.556 μg/mL, 1.419 μg/mL, 0.209 μg/mL, 9.339 μg/mL and 5.371 μg/mL, respectively, which were far less than the positive control acarbose (IC50 355.4 μg/mL). In addition, the kinetic analysis showed the inhibitory effects of the four compounds on α-glucosidase were competitive, and the Ki were 27.18 μg/mL, 2.06 μg/mL, 8.42 μg/mL and 19.58 μg/mL, respectively. Therefore, sweet potato leaf extract has significant α-glucosidase inhibition, and could provide a theoretical basis for sweet potato leaves as a resource of hypoglycemic drug development or health care.
