Investigation of Antibiotic Resistance and Biofilm-forming Ability in Staphylococcus aureus
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Abstract:
Staphylococcus aureus (S. aureus) has been long recognized as a common food-borne pathogen in food safety. To study their individuality, drug resistance and biofilm-forming capacity, a total of 127 Staphylococci strains were subject to the investigation. Preliminary identification of Staphylococcus aureus was conducted with a rapid confirmatory latex agglutination test kit and API staph test strips. S.aureus was further confirmed with the detection of specific 16S rRNA and femA genes by PCR. The molecular method was also used for amplification of mecA and orfX elements to identify meticillin-resistence S. aureus (MRSA). The biofilm-forming capacity of 127 clinical isolates of S. aureus was tested using a polystyrene 96-well plate format and crystal violet staining. In this experiment, 127 staphylococci were isolated, in which 107 strains were MRSA, 12 strains belonged to meticillin-sensitive S. aureus (MSSA) and 6 strains contributed to methicillin-resistant coagulase-negative staphylococci (MRCNS). All staphylococci isolates formed biofilms, and 75 strains (59.1%) belonged to the weak adhesive group. The molecular method was more accurate and rapid for the identification of strains. The majority of the strains formed weak adhesive biofilms and there was no significant relationship between the genotype of drug resistance and biofilm formation ability.
