Effects of Simplified Defined Medium on Nitrite Degradation by Lactobacillus plantarum
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Abstract:
Lactobacillus plantarum L134-1-P (LP) was investigated in the simplified defined mediums to understand its nutritional requirements and trying to find out nitrite degradation mechanism,. It was found that nitrite was barely degraded by LP in blank control. Growth and nitrite reduction activity were restrained in mediums with 3% carbon source (glucose, sucrose and corn starch), the final degradation rate was only 6.70% after 72 h. When adding 3% glucose and 2% nitrogen source, the pH of yeast extract medium, enzymatic and non-enzymatic hydrolysis isolated soy protein mediums were 3.84, 3.89 and 4.64 respectively, with nitrite degradation rate of 96.44%, 96.27% and 36.44 respectively, after 72 h. And the final pH and nitrite degradation rate of ammonium nitrate medium were only 5.27 and 8.34%. On that basis, pH of lactic acid solution was adjusted to 4 and 3, the degradation rates of nitrite were 31.33% and 81.30%, respectively. In short, the degradation course relied on enzymolysis and acid splitting when enough nutrition was offered. And acid splitting and enzymolysis could be reinforced when LP was afforded to ferment solution under pH of 4.
