Multiplex PCR Assays for Escherichia coli Phylogenetic Assignments and Virulence Gene Ownership
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Abstract:
The relationship between different phylogroups and virulence genes of E.coli from various origins were identified. Forty-seven virulence genes (VGs) markers found in six pathotypes of E. coli known to cause intestinal and extra-intestinal disease in humans or animals were investigated. Multiplex PCR assays were used to screen 42 strains including clinical and commensal isolates for these virulence genes and assign the strains to phylogenetic group. Results showed that IPEC isolates possessed typical IPEC genes and some ExPEC genes. The non-pathogenic strains also possessed ExPEC genes. None of the ExPEC or non-pathogenic isolates harbored VGs typical for IPEC. The phylogenetic grouping of 42 isolates showed that most of the ExPEC strains belong to group B2 or D. Most of the intestinal clinical isolates were confined to groups D (19.2%), B1 (50.0%) and A (30.8%), Three non-pathogenic strains, DH5α,BL-21 and XL-10, belonged to group A. This study indicated that the presence of virulence genes were closely related to phylogenetic groupings.
