Screening and Diversity Analysis of Antimicrobial Activity Bacteria Associated with Sargassum integerrimum from Naozhou Island
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Abstract:
Antimicrobial activity bacteria associated with Sargassum integerrimum were isolated by using plate pouring method. Their antimicrobial activities were determined by Oxford cup method in which Escherichia coli, Staphylococcus aurous and Candida albicans were used as representative indicators for gram-negative bacteria, gram-negative bacteria and fungi, respectively. The antimicrobial activity strains were then identified according to morphological characteristics, biochemical properties and the phyogenetical analysis of 16S rRNA sequence. The diversity of antimicrobial activity isolates was also analyzed. Results indicated that 36 symbiotic bacteria were isolated, 11 of which had antimicrobial activity The subsequent analysis showed that all of the isolates belonged to five genera (Bacillus, Staphylococcu, Kocuria, Microbacterium, Brachybacterium) of five families (Bacillaceae, Staphylococcaceae, Micrococcaceae, Microbacteriaceae, Dermabacteraceae) in two phylogenetic groups (Firmicutes, Actinobacteria) and among them Firmicutes was their dominant phylum(7 strains, 63.6 %). In addition, four strains with potential new species (X24, X31, X21 and X34) were found. It is suggested that Sargassum integerrimum from Naozhou Island is abundant in the bacteria with antimicrobial activity and more potential new species could be collected.
