Mechanism of Porcine Collagen Type Ⅱ and Collagen Peptide on Anti-arthritis
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Abstract:
Collagen was extracted from pig cartilage by salting out, and collagen peptide was hydrolyzed by alkaline protease. Collagen purity and the physicochemical properties of collagen and peptide were analyzed. The effect of anti-arthritic was studied by constructing CIA model. 82.86% of collagen was obtained, with molecular weight of more than 300 KD.The maximum absorption peak of UV was at 230 nm, and no obvious absorption peak was observed at 280 nm. Therefore the extracted collagen possessed the characteristics of natural collagen type Ⅱ, while the molecular weight of collagen peptide was less than 20 KD and no collagen type Ⅱ was observed. Collagen type Ⅱ could reduce index score and joint deformation rate, and significantly reduce IL-17, MMP-3 and TNF-α levels. In addition, Collagen type Ⅱ could significantly increase the T cell stimulation index (P＜0.05), but had no effect on B cell stimulation index (p＞0.05). Tissue sections showed that Collagen type Ⅱ could reduce destruction of joint and inhibit cartilage degradation. However, no such funcions were found with collagen peptide .
