Optimization of Ultrasonic Extraction and Physiochemical Characteristics of Polysaccharides from Sophora alopecuroides Seeds
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Abstract:
Response surface methodology (RSM) was used to optimize the ultrasonic extraction process of polysaccharides from Sophora alopecuroides seeds. RSM was employed to evaluate the interactive effects and significance of four crucial variables including ultrasonic extraction time, ultrasonic power, extraction temperature and ratio of water to seed, mathematical model for the yield of polysaccharides from Sophora alopecuroides seeds as functions was established. The optimum conditions were: ultrasonic extraction time 30 min, ultrasonic power 280 W, extraction temperature 50 ℃, water to seed ratio 50:1. Under the optimized conditions, the yield was up to 8.51%, which was in close agreement with value predicted by the model. Compared with traditional hot water extraction, the ultrasonic extraction shortened extraction time, lowered extraction temperature and increased extraction yield. The structural properties of polysaccharides were preliminarily analyzed by IR spectroscopy, UV spectra and gas chromatograph (GC), and the physiochemical properties of polysaccharides were determined.
