Abstract:As a kind of agricultural waste, cotton seed husk is a potential source of functional xylooligosaccharides. In this study, a new strain NS5, which could produce endo-xylanase, was screened by France Biomerieux microorganism automatic identification system and preliminarily identified as Sphingomonas paucimobilis. The optimum enzymolysis conditions for xylooligosaccharides production were obtained as follows: temperaure 30 ℃, time 8 h, xylanase concentration 15 % and xylan concentration 40 g/L. Under these conditions, the yield of xylooligosaccharides was 53.20%. By analysis with HPLC, xylooligosaccharides was accounted for more than 82% of the total sugar, 48.56 % of which were identified as xylobiose and xylotriose. These results provided information for xylooligosaccharides preparation from cotton seed husk for future use in food industry.
