Enzymes Screening for Preparation of High-glutamine Oligopeptide from Gluten
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Abstract:
To obtain high-glutamine oligopeptide, eight kinds of proteases including Pancreatin, Alcalase, Neutrase, Pepsin, Acidic protease, Protamex and Flavourzyme were used during gluten hydrolysis process. The releasing characteristic of glutamine peptide was analyzed. Alcalase and Protamex were screened as the best hydrolytic enzyme. Compared with other enzymes, Alcalase and Protamex gave the effective hydrolysis and minimized protease damaging effects of amide groups, with the effective product of glutamine content up to 20%, and molecular weight below 3000 Da was up to 95%. In particularly, 75% of Alcalase hydrolysates had molecular weight less than 1000 Da. Compared with previous studies, the molecular weight distribution was used as an important basis to choose protease instead of DH, which can accurately reflect the features of enzymatic hydrolysis and avoid interference with free amino acid.
