Anti-oxidative Damage of Portulace oleracea Polysaccharide on Reproductive System in Male Rats
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Abstract:
The protection mechanism of Portulace Oleracea polysaccharide (POP) on reproductive toxicity and oxidative damage induced by EEs in male rats was studied to provide the experimental basis for development and application of POP. The male rats were established as fertility problems model by intraperitoneal injecting ethinyloestradiol (EE2), with the injecting dose of 0.1 mg/kg, 0.5 mg/kg, 1 mg/kg and 5 mg/kg, and meanwhile intraperitoneal injection of 10% POP as the comparative group.Absolute and relative testicular weights were measured. Morphological changes of testicular were observed in the light microscope. The contents of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxide capacity (T-AOC), malondialdehyde (MDA) and catalase (CAT) in testicular tissue and plasma were detected. In EE2 processed groups, testicular weight decreased significantly (P<0.05) and led to shrink when injection of 1mg/kg EE2. The antioxidant enzymes including SOD, GSH-Px, CAT and T-AOC in testicular tissue all decreased (P<0.05) which resulted in the increase of MDA (P<0.05) with the increase of EE2 dosage. On the contrary, SOD, GSH-Px, CAT, T-AOC and MAD were unchanged (P>0.05) in POP group. EE2 decreased antioxidase activity and closely related to the content of reactive oxygen species (ROS). And POP had the effect of anti-oxidative damage, protected the balance of ROS and the defense system of antioxidant enzyme.
