Determination of Five Mycotoxins in Peanuts and Products by HPLC-MS/MS
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Abstract:
A method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for simultaneous determination of five mycotoxins, namely aflatoxin B1 (AFB1) , aflatoxin M1 (AFM1), Deoxynivalenol (DON), Zearalenone (ZEA) and OchratoxinA (OTA), in peanut and its products. The samples were extracted with methanol-aqueous solution (55:45, V/V), cleaned up and enriched by mycotoxin affinity column, and then analyzed by HPLC-MS/MS under the multiple reaction monitoring (MRM) mode. All the analytes were successfully detected in positive ion mode within 6 min, and the correlation coefficients (r2, n=6) were more than 0.999. The linear ranges for AFB1, AFM1, and DON were between 0.5 μg/L to 50.0 μg/L, with detection limit of (LOD, S/N=3) 0.05 μg/kg. While ZEA and OTA were ranging from 5.0 μg/L to 100.0 μg/L, and the detection limit (LOD, S/N=3) was 0.5 μg/kg. The spiked recoveries were in the range of 86.8~102.7%, and relative standard deviations (RSD, n=3) were 0.36~4.79%. The method was simple, effective and sensitive, and was suitable for the determination and confirmation of five mycotoxins in peanuts and products samples.
